| First Author | Rikke BA | Year | 1991 |
| Journal | Genomics | Volume | 11 |
| Issue | 4 | Pages | 895-904 |
| PubMed ID | 1783397 | Mgi Jnum | J:1867 |
| Mgi Id | MGI:50391 | Doi | 10.1016/0888-7543(91)90012-4 |
| Citation | Rikke BA, et al. (1991) LINE-1 repetitive DNA probes for species-specific cloning from Mus spretus and Mus domesticus genomes. Genomics 11(4):895-904 |
| abstractText | Mus domesticus and Mus spretus mice are closely related subspecies. For genetic investigations involving hybrid mice, we have developed a set of species-specific oligonucleotide probes based on the detection of LINE-1 sequence differences. LINE-1 is a repetitive DNA family whose many members are interspersed among the genes. In this study, library screening experiments were used to fully characterize the species specificity of four M. domesticus LINE-1 probes and three M. spretus LINE-1 probes. It was found that the nucleotide differences detected by the probes define large, species-specific subfamilies. We show that collaborative use of such probes can be employed to selectively detect thousands of species-specific library clones. Consequently, these probes could be exploited to monitor and access almost any given species-specific region of interest within hybrid genomes. |
