| First Author | Aono S | Year | 2000 |
| Journal | J Biol Chem | Volume | 275 |
| Issue | 1 | Pages | 337-42 |
| PubMed ID | 10617623 | Mgi Jnum | J:59346 |
| Mgi Id | MGI:1351413 | Doi | 10.1074/jbc.275.1.337 |
| Citation | Aono S, et al. (2000) Genomic organization and expression pattern of mouse neuroglycan C in the cerebellar development. J Biol Chem 275(1):337-42 |
| abstractText | Neuroglycan C (NGC) is a membrane-spanning chondroitin sulfate proteoglycan with an epidermal growth factor module that is expressed predominantly in the brain. Cloning studies with mouse NGC cDNA revealed the expression of three distinct isoforms (NGC-I, -II, and -III) in the brain and revealed that the major isoform showed 94. 3% homology with the rat counterpart. The NGC gene comprised six exons, was approximately 17 kilobases in size, and was assigned to mouse chromosome band 9F1 by fluorescence in situ hybridization. Western blot analysis demonstrated that, although NGC in the immature cerebellum existed in a proteoglycan form, most NGC in the mature cerebellum did not bear chondroitin sulfate chain(s), indicating that NGC is a typical part-time proteoglycan. Immunohistochemical studies showed that only the Purkinje cells were immunopositive in the cerebellum. In the immature Purkinje cells, NGC, probably the proteoglycan form, was immunolocalized to the soma and thick dendrites on which the climbing fibers formed synapses, not to the thin branches on which the parallel fibers formed synapses. This finding suggests the involvement of NGC in the differential adhesion and synaptogenesis of the climbing and parallel fibers with the Purkinje cell dendrites. |
