| First Author | Kronmiller JE | Year | 1995 |
| Journal | Arch Oral Biol | Volume | 40 |
| Issue | 9 | Pages | 831-8 |
| PubMed ID | 8651887 | Mgi Jnum | J:29483 |
| Mgi Id | MGI:77015 | Doi | 10.1016/0003-9969(95)00053-r |
| Citation | Kronmiller JE, et al. (1995) Spatial and temporal distribution of sonic hedgehog mRNA in the embryonic mouse mandible by reverse transcription/polymerase chain reaction and in situ hybridization analysis. Arch Oral Biol 40(9):831-8 |
| abstractText | Hedgehog genes have recently been implicated in the control of pattern formation in many developing organ system. Vertebrate homologues of the Drosophila hedgehog have been identified in mouse and rate embryos. The temporal regulation of sonic hedgehog (mouse homologue) has previously been studied by Northern analysis of whole embryos with varying results. Sonic hedgehog transcript expression in the mouse mandibular process was now characterized using polymerase chain reaction (PCR) an in situ hybridization techniques. PCR analysis revealed transcripts at gestational days 10 and 11, before the formation of the dental lamina, but not at days 12-14, after tooth buds have formed. Transcripts were localized to, primarily, the epithelium in the presumptive incisor region of the mandibular midline at gestational day 10. No mRNA was detected by in situ hybridization techniques in the presumptive molar regions of odontogenic epithelium. Sonic hedgehog expression may be involved in the regulation of pattern formation through establishment of an incisor-molar axis of polarity. |
