| First Author | Li X | Year | 2017 |
| Journal | Cell Stem Cell | Volume | 21 |
| Issue | 2 | Pages | 264-273.e7 |
| PubMed ID | 28648365 | Mgi Jnum | J:253977 |
| Mgi Id | MGI:6101292 | Doi | 10.1016/j.stem.2017.05.019 |
| Citation | Li X, et al. (2017) Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State. Cell Stem Cell 21(2):264-273.e7 |
| abstractText | Direct lineage reprogramming, including with small molecules, has emerged as a promising approach for generating desired cell types. We recently found that during chemical induction of induced pluripotent stem cells (iPSCs) from mouse fibroblasts, cells pass through an extra-embryonic endoderm (XEN)-like state. Here, we show that these chemically induced XEN-like cells can also be induced to directly reprogram into functional neurons, bypassing the pluripotent state. The induced neurons possess neuron-specific expression profiles, form functional synapses in culture, and further mature after transplantation into the adult mouse brain. Using similar principles, we were also able to induce hepatocyte-like cells from the XEN-like cells. Cells in the induced XEN-like state were readily expandable over at least 20 passages and retained genome stability and lineage specification potential. Our study therefore establishes a multifunctional route for chemical lineage reprogramming and may provide a platform for generating a diverse range of cell types via application of this expandable XEN-like state. |
