| First Author | Leonard PH | Year | 2015 |
| Journal | BMC Dev Biol | Volume | 15 |
| Pages | 23 | PubMed ID | 26021315 |
| Mgi Jnum | J:222236 | Mgi Id | MGI:5644179 |
| Doi | 10.1186/s12861-015-0073-x | Citation | Leonard PH, et al. (2015) The Aurora A-HP1gamma pathway regulates gene expression and mitosis in cells from the sperm lineage. BMC Dev Biol 15:23 |
| abstractText | BACKGROUND: HP1gamma, a well-known regulator of gene expression, has been recently identified to be a target of Aurora A, a mitotic kinase which is important for both gametogenesis and embryogenesis. The purpose of this study was to define whether the Aurora A-HP1gamma pathway supports cell division of gametes and/or early embryos, using western blot, immunofluorescence, immunohistochemistry, electron microscopy, shRNA-based knockdown, site-directed mutagenesis, and Affymetrix-based genome-wide expression profiles. RESULTS: We find that the form of HP1gamma phosphorylated by Aurora A, P-Ser83 HP1gamma, is a passenger protein, which localizes to the spermatozoa centriole and axoneme. In addition, disruption in this pathway causes centrosomal abnormalities and aberrations in cell division. Expression profiling of male germ cell lines demonstrates that HP1gamma phosphorylation is critical for the regulation of mitosis-associated gene expression networks. In female gametes, we observe that P-Ser83-HP1gamma is not present in meiotic centrosomes of M2 oocytes, but after syngamy, it becomes detectable during cleavage divisions, coinciding with early embryonic genome activation. CONCLUSIONS: These results support the idea that phosphorylation of HP1gamma by Aurora A plays a role in the regulation of gene expression and mitotic cell division in cells from the sperm lineage and in early embryos. Combined, this data is relevant to better understanding the function of HP1gamma in reproductive biology. |
