| Primary Identifier | MGI:5051626 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Ptgds |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | A targeting vector was constructed with the nls-cre recombinase sequence fused to exon 1 of the Ptgds locus and a poly A signal with a floxed EGFP-hygromycin selection cassette 3' to the cre poly A signal. Homologous recombination in ES cells was performed to replace exon 1 with the targeting construct. Chimeras were crossed with FVB/N mice. The floxed selection cassette was excised by crossing offspring to the Tg(EIIa-cre)C5379Lmgd deletor line and the EIIa transgene was bred from the background. |
