| Primary Identifier | MGI:5920009 | Allele Type | Transgenic |
| Attribute String | Conditional ready, Humanized sequence, Inserted expressed sequence | Gene | Tg(tetO-TARDBP*A315T)#Lmit |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The human cDNA was engineered with a c.1077G>A point mutation, resulting in codon 315 changing from alanine to threonine (p.A315T or p.Ala315Thr). This mutation is associated with familial amyotrophic lateral sclerosis (ALS) in humans. A construct comprising the tetracycline inducible tetO promoter, the human cDNA and an SV40 poly signal, was injected into the pronuclei of fertilized C57BL/6 oocytes. Two lines, numbers 6 and 13, were selected for further study. |
