Primary Identifier | MGI:3574670 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Rps6ka4 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exons 5-7, which encode amino acids 155-251 and make up the middle of the N-terminal kinase domain, were replaced with a neo. If readthrough occurred, splicing from exons 4-8 would result in a frameshift mutation. Immunoblotting of mutant MEFs confirmed the lack of protein. |