Primary Identifier | MGI:7571453 | Allele Type | Endonuclease-mediated |
Attribute String | Not Specified | Gene | Otof |
Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NCrl |
Is Recombinase | false | Is Wild Type | false |
molecularNote | This mouse line was generated by site-directed CRISPR-Cas9 mutagenesis in C57BL/6NCrl mice causing a D1821A/D1822A mutation (Corresponds to D1841A/D1842A mutation in the isoform e peptide coded for by the human ortholog). Additional silent mutations were introduced. The mutated exon38 now reads:(5')gtggctgaagggccagcaggaggacaaacaggacacagatgtccactatcactccctcacgggggagggcaacttcaactggagatacctcttccccttcgactacctagcggccgaagagaagatcgttatgtccaaaaaggagtctatgttctcctgggatgagacggagtacaagatccctgcgcggctcaccctgcagatctgggacgctgaTcacttctcggctgCTgCcttcctgg (3') |