| First Author | Marzban G | Year | 2002 |
| Journal | Exp Gerontol | Volume | 37 |
| Issue | 12 | Pages | 1451-60 |
| PubMed ID | 12559414 | Mgi Jnum | J:81158 |
| Mgi Id | MGI:2448186 | Doi | 10.1016/s0531-5565(02)00117-1 |
| Citation | Marzban G, et al. (2002) Age-related alterations in the protein expression profile of C57BL/6J mouse pituitaries. Exp Gerontol 37(12):1451-60 |
| abstractText | The aim of our study was to monitor the protein expression profile in pituitary glands of healthy C57BL/6J mice during aging. Pituitary glands of 4-week old (immature), 3-month old (mature), and >25-month old mice were analysed by proteomic tools such as two-dimensional electrophoresis and N-terminal micro-sequencing. A change was detected in the expression of growth hormone after sexual maturation. Our particular interest, however, was directed against up-regulated proteins in the old pituitary glands, which are proposed to be involved in the process of neuroendocrine aging.Among these proteins, the expression of glutathione-S-transferase (GST) and apolipoprotein A-1 were increased in old pituitaries. Furthermore, ubiquitin carboxyl-terminal hydrolase (UCH-L1) was significantly up-regulated in senescent C57BL/6J mouse pituitaries. Since only the rat homologue was known, we isolated and analysed the mouse UCH-L1 sequence. Since GST is involved in antioxidative defence and UCH-L1 is part of the ubiquitin/proteasome system, which is responsible for the removal of damaged proteins, these results suggest increased oxidative burden and an increased activity of the ubiquitin system. |
