| First Author | Mayo MW | Year | 1994 |
| Journal | Oncogene | Volume | 9 |
| Issue | 7 | Pages | 1999-2008 |
| PubMed ID | 8208545 | Mgi Jnum | J:18873 |
| Mgi Id | MGI:67095 | Citation | Mayo MW, et al. (1994) Phorbol esters support the proliferation of a hematopoietic cell line by upregulating c-jun expression. Oncogene 9(7):1999-2008 |
| abstractText | FD/PMA, a derivative of the interleukin-3 (IL-3) dependent FDC-P1 cell line, proliferates in response to either phorbol esters (phorbol 12-myristate 13-acetate, PMA) or IL-3. Analysis of immediate-early gene expression revealed that FD/PMA cells contained elevated levels of c-jun transcripts when grown in the presence of phorbol esters. Upregulation of c-jun mRNA was specific since other jun family members (namely jun-B and jun-D) displayed similar patterns of gene expression as observed in the parental cell line. The accumulation of c-jun RNA was due to increased c-jun transcription and not the result of altered message stability. Furthermore, elevated c-jun expression resulted in increased AP-1 binding activity in FD/PMA cells. Antisense c-jun oligonucleotides suppressed proliferation of FD/PMA cells by 80% and resulted in a significant reduction in both AP-1 binding activity and c-jun message levels. Collectively, these results demonstrate that FD/PMA cells require elevated levels of c-jun expression for PMA-responsive proliferation and indicate that tumor promoters have the ability to abrogate IL-3 dependency by elevating AP-1 activity. |
