| First Author | Drexler K | Year | 1993 |
| Journal | Immunol Lett | Volume | 37 |
| Issue | 2-3 | Pages | 187-96 |
| PubMed ID | 8258459 | Mgi Jnum | J:15115 |
| Mgi Id | MGI:63252 | Doi | 10.1016/0165-2478(93)90030-6 |
| Citation | Drexler K, et al. (1993) Limited heterogeneity of T-cell receptor V beta gene expression in the early stage of insulitis in NOD mice. Immunol Lett 37(2-3):187-96 |
| abstractText | Non-obese-diabetic (NOD) mice spontaneously develop type I diabetes. The disease starts with T cells infiltrating the islets of Langerhans. We therefore examined the T-cell receptor (TCR) V beta region repertoire in islet infiltrates from individual female NOD mice from 4 to 10 week old by polymerase chain reaction (PCR) using V beta 1-V beta 17 specific oligonucleotides. The study revealed a limited heterogeneity of TCR V beta transcripts with a predominance of V beta 1 at the onset of insulitis, i.e. at 4 weeks of age. The TCR VDJ beta sequences of the V beta 1 PCR fragments were identical in most of the individual mice. Among several different mice, similarities in the beta-junctional regions were detected. In contrast, a large heterogeneity of TCR V beta usage was found in mice with advanced insulitis, i.e., from 6 weeks of age on. Thus, these data suggest a limited heterogeneity of TCR V beta usage with a predominance of V beta 1 at the initiation phase of the disease. |
