| First Author | Yuan A | Year | 2003 |
| Journal | J Neurosci | Volume | 23 |
| Issue | 28 | Pages | 9452-8 |
| PubMed ID | 14561875 | Mgi Jnum | J:86249 |
| Mgi Id | MGI:2679157 | Doi | 10.1523/JNEUROSCI.23-28-09452.2003 |
| Citation | Yuan A, et al. (2003) Neurofilament transport in vivo minimally requires hetero-oligomer formation. J Neurosci 23(28):9452-8 |
| abstractText | Neurofilament assembly requires at minimum the polymerization of neurofilament light chain (NF-L) with either neurofilament medium chain (NF-M) or neurofilament heavy chain (NF-H) subunits, but requirements for their axonal transport have long been controversial. Using a gene deletion approach, we generated mice containing only NF-L or NF-M. In vivo pulse radiolabeling analyses in retinal ganglion cell neurons revealed that NF-L alone is incapable of efficient transport, whereas nearly one-half of the normal level of NF-M is transported along optic axons in the absence of the other triplet subunits. Under these conditions, however, NF-M transport is completely abolished by deleting alpha-internexin. Our results strongly suggest that efficient neurofilament protein transport in vivo minimally requires hetero-oligomer formation. They also show that NF-M can partner with intermediate filament proteins other than the NF-H and NF-L subunits in neurons to support slow transport and possibly other functions of neuronal intermediate filaments. |
