| First Author | Taylor KD | Year | 1991 |
| Journal | Mol Reprod Dev | Volume | 28 |
| Issue | 4 | Pages | 319-24 |
| PubMed ID | 2064774 | Mgi Jnum | J:11298 |
| Mgi Id | MGI:59737 | Doi | 10.1002/mrd.1080280402 |
| Citation | Taylor KD, et al. (1991) Expression of the rig gene in mouse oocytes and early embryos. Mol Reprod Dev 28(4):319-24 |
| abstractText | A clone selected from a two-cell mouse embryo cDNA library has been sequenced and identified as rig cDNA. The rig gene codes for a highly conserved nuclear protein, which may have a general role in cell growth or replication (Shiga et al.: Proc Natl Acad Sci USA 87:3594, 1990). The quantitative changes in rig mRNA were studied in blot hybridization experiments with total RNA from oocytes and early embryos. The amount and relative abundance of rig mRNA change considerably during early development. There are about 1.6 x 10(4) rig mRNA molecules in a late growth-stage oocyte; this number is reduced to about one-tenth in the ovulated egg but increases about twenty-fold during cleavage through the blastocyst stage. In F9 embryonal carcinoma cells, the relative abundance of rig mRNA is similar to that in blastocysts (about 0.1% of the mRNA population), but it is about eight-fold higher in the mouse myeloma cell line MOPC-104E. The high level of rig mRNA in late growth-stage oocytes suggests that the rig gene product may be important for overall transcriptional activity rather than DNA replication and mitosis. Alternatively, the rig protein may be a storage product of oogenesis and have a role in the initiation of development. |
