| First Author | Cazalla D | Year | 2005 |
| Journal | Mol Cell Biol | Volume | 25 |
| Issue | 8 | Pages | 2969-80 |
| PubMed ID | 15798186 | Mgi Jnum | J:97604 |
| Mgi Id | MGI:3575934 | Doi | 10.1128/MCB.25.8.2969-2980.2005 |
| Citation | Cazalla D, et al. (2005) A novel SR-related protein is required for the second step of Pre-mRNA splicing. Mol Cell Biol 25(8):2969-80 |
| abstractText | The SR family proteins and SR-related polypeptides are important regulators of pre-mRNA splicing. A novel SR-related protein of an apparent molecular mass of 53 kDa was isolated in a gene trap screen that identifies proteins which localize to the nuclear speckles. This novel protein possesses an arginine- and serine-rich domain and was termed SRrp53 (for SR-related protein of 53 kDa). In support for a role of this novel RS-containing protein in pre-mRNA splicing, we identified the mouse ortholog of the Saccharomyces cerevisiae U1 snRNP-specific protein Luc7p and the U2AF65-related factor HCC1 as interacting proteins. In addition, SRrp53 is able to interact with some members of the SR family of proteins and with U2AF35 in a yeast two-hybrid system and in cell extracts. We show that in HeLa nuclear extracts immunodepleted of SRrp53, the second step of pre-mRNA splicing is blocked, and recombinant SRrp53 is able to restore splicing activity. SRrp53 also regulates alternative splicing in a concentration-dependent manner. Taken together, these results suggest that SRrp53 is a novel SR-related protein that has a role both in constitutive and in alternative splicing. |
