| First Author | Mandasari M | Year | 2016 |
| Journal | Biochem Biophys Res Commun | Volume | 469 |
| Issue | 3 | Pages | 761-7 |
| PubMed ID | 26682927 | Mgi Jnum | J:233171 |
| Mgi Id | MGI:5780914 | Doi | 10.1016/j.bbrc.2015.12.006 |
| Citation | Mandasari M, et al. (2016) A facile one-step strategy for the generation of conditional knockout mice to explore the role of Notch1 in oroesophageal tumorigenesis. Biochem Biophys Res Commun 469(3):761-7 |
| abstractText | NOTCH1 plays an important role in epithelial differentiation and carcinogenesis. To investigate the impact of Notch1 inactivation in oroesophageal epithelium, we generated conditional knockout (cKO) mice, using a combined construct which induces the expression of single guide RNA targeting Notch1 and Cas9 by the KRT14 promoter. The cKO mice exhibited patchy hair loss and multiple NOTCH1-negative areas in the tongue epithelium, indicative of heterogeneous knockout. The cKO mice showed susceptibility to esophageal tumorigenesis, underscoring Notch1 as a tumor suppressor. Our one-step strategy for generation of cKO mice provides a versatile method to examine a gene function in vivo. |
