| First Author | Kataoka T | Year | 2002 |
| Journal | Exp Cell Res | Volume | 273 |
| Issue | 2 | Pages | 256-64 |
| PubMed ID | 11822881 | Mgi Jnum | J:74813 |
| Mgi Id | MGI:2159137 | Doi | 10.1006/excr.2001.5438 |
| Citation | Kataoka T, et al. (2002) Expression level of c-FLIP versus Fas determines susceptibility to Fas ligand-induced cell death in murine thymoma EL-4 cells. Exp Cell Res 273(2):256-64 |
| abstractText | The caspase-8 inhibitor c-FLIP blocks death receptor-mediated cell death and plays an essential role in the regulation of lymphocyte homeostasis and the immune escape of tumors. The murine thymoma cell line EL-4 was resistant to Fas ligand (FasL)-induced apoptosis by constitutive expression of FLIP (L). Cycloheximide downregulated the expression of FLIP (L) and markedly sensitized EL-4 cells to FasL-induced apoptosis. In contrast, DNA-damaging agents sensitized EL-4 cells to FasL-induced cell death via an increase of cell-surface Fas without any influence on FLIP (L) expression. Enforced expression of transfected Fas rendered EL-4 cells highly susceptible to FasL-induced cell death. These findings demonstrate that susceptibility to FasL-induced cell death mainly depends on the expression level of c-FLIP versus cell-surface Fas. |
