| First Author | Wei J | Year | 2013 |
| Journal | Mol Immunol | Volume | 55 |
| Issue | 3-4 | Pages | 303-9 |
| PubMed ID | 23522925 | Mgi Jnum | J:202144 |
| Mgi Id | MGI:5517536 | Doi | 10.1016/j.molimm.2013.03.004 |
| Citation | Wei J, et al. (2013) MyD88 as a target of microRNA-203 in regulation of lipopolysaccharide or Bacille Calmette-Guerin induced inflammatory response of macrophage RAW264.7 cells. Mol Immunol 55(3-4):303-9 |
| abstractText | MicroRNAs (miRNAs) have been demonstrated to play a pivotal role in the regulation of target gene expression at the post-transcriptional level. In order to better understand the role of microRNA-203 (miR-203) in the immunological regulation, the function of miR-203 was explored in the macrophage RAW264.7 cells against lipopolysaccharide (LPS) or Bacille Calmette-Guerin (BCG) stimulation. The results evidenced that myeloid differentiation factor 88 (MyD88) was a novel target of miR-203, miR-203 was capable of directly targeting the 3' untranslated region (3'UTR) of MyD88 and post-transcriptionally down-regulating the expression of protein. In addition, an overexpression of miR-203 in RAW264.7 cells was correlated with repressions of MyD88, as well as its downstream signaling of NF-kappaB (NF-kappaB1), TNF-alpha and IL-6. These results suggest that miR-203 may be an important regulator in macrophages against LPS or mycobacteria infection, which may through a mechanism of negatively regulating MyD88-dependent Toll-like receptors signaling pathway. |
