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Publication : Mouse MCM proteins: complex formation and transportation to the nucleus.

First Author  Kimura H Year  1996
Journal  Genes Cells Volume  1
Issue  11 Pages  977-93
PubMed ID  9077461 Mgi Jnum  J:49048
Mgi Id  MGI:1276618 Doi  10.1046/j.1365-2443.1996.840284.x
Citation  Kimura H, et al. (1996) Mouse MCM proteins: complex formation and transportation to the nucleus. Genes Cells 1(11):977-93
abstractText  BACKGROUND: The members of the MCM protein family, including MCM2, MCM3, Cdc21, CDC46, Mis5 and CDC47, are considered to be involved in the control of a single round of DNA replication during S phase in eukaryotes. They bind to chromatin during G1 and detach from it during S phase as if they license the chromatin to replicate. However, unlike the originally proposed 'licensing factor' and the budding yeast homologues, mammalian MCM2 and P1MCM3 proteins appeared to be localized in the nucleus during the interphase. RESULTS: We purified mCdc21 and its associated proteins from mouse cell extract by anti-mCdc21 immunoaffinity chromatography. Three proteins which co-purified with mCdc21 were identified as mCDC47, mMis5 and mMCM2, all were MCM proteins. Glycerol gradient centrifugation analysis showed that all the mouse MCM proteins were detected at 450-600 kDa, an indication of the sum of their calculated molecular weights from their amino acid sequences. mCdc21 was displaced from replicated chromatin in a similar way to P1MCM3 and MCM2 during S phase. Among the six mouse MCM proteins, only mMCM2 and mP1MCM3 showed nuclear localization when overexpressed in COS cells. CONCLUSIONS: We conclude that in the mouse, six MCM proteins form a single protein complex of molecular weight 450- 600 kDa, which may enter the nucleus by nuclear localization signals in the mMCM2 and mP1MCM3 subunits.
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