| Primary Identifier | MGI:5544431 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Spink1 |
| Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | A loxJTZ17 site was inserted at the 5' end of a cassette containing the NLS-cre-polyA sequence (nuclear localization signal-cre recombinase-polyadenylation signal). A PGK-puromycin selection cassett and a lox2272 site was inserted at the 3' end of the targeting construct. Targeted recombination in ES cells already containing Spink3tm1Kymm inserted the cre cassette upstream of exon 1 of Spink3, replacing the neo marker. Chimeras were produced from correctly targeted ES cell clones and germline transmission was established by crosses to C57BL6/J animals. |
