| Primary Identifier | MGI:2183359 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Tbp |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Animals heterozygous for Tbptm1Ees were crossed to a cre-expressing line to facilitate excision of the neomycin resistance cassette. The resulting chimeric protein is composed of amino acids 1-24 followed by 2 tandem repeats of the FLAG tag and the entire C-terminus (amino acids 136-316). RNase protection assay and Western blot analysis confirmed that gene expression and steady-state accumulation of the protein in homozygous mutant animals is similar to that of the wild-type protein. |
