| First Author | Bumsted-O'Brien KM | Year | 2007 |
| Journal | J Comp Neurol | Volume | 505 |
| Issue | 1 | Pages | 58-72 |
| PubMed ID | 17729288 | Mgi Jnum | J:132519 |
| Mgi Id | MGI:3776195 | Doi | 10.1002/cne.21458 |
| Citation | Bumsted-O'Brien KM, et al. (2007) Expression of the homeodomain transcription factor Meis2 in the embryonic and postnatal retina. J Comp Neurol 505(1):58-72 |
| abstractText | Members of the Meis subfamily of homeodomain-containing transcription factors play important roles during development and disease. Here we report that the Meis family protein Meis2 is expressed by a subpopulation of gamma-aminobutyric acid (GABA)ergic amacrine (AM) cells in the adult and embryonic retina of different vertebrate species. In mice, Meis2-expressing (Meis2+) AM cells are not cholinergic or dopaminergic, but some are immunoreactive for neuronal nitric oxide synthase (bNOS). About 50% of the mouse Meis2+ AM cell population expresses the calcium-binding protein calretinin, and some Meis2+ AM cells show characteristics of Type II CD-15+ cells. AM cell expression of Meis2 is lost in a conditional knockout mouse model for Pax6, indicating a dependency upon Pax6. Bromodeoxyuridine pulse labeling experiments and immunohistochemical staining for the neuronal marker NeuN in embryonic mouse retinae indicate that Meis2 is an early marker for newly postmitotic AM cells. In addition, taking advantage of the protracted retinal development in humans, we show that newly generated AM cells express Meis2 before adopting the GABAergic or glycinergic neurotransmitter phenotype. As development proceeds, some AM cells lose Meis2 expression concomitantly with the appearance of glycine, while other AM cells retain Meis2 expression after they express GABA. These data identify Meis2 as a suitable marker for the study of AM cell diversity and development in addition to providing evidence for the stepwise specification of the glycinergic and GABAergic neurotransmitter phenotypes during AM cell differentiation. |
