| First Author | van Duin M | Year | 1988 |
| Journal | Nucleic Acids Res | Volume | 16 |
| Issue | 12 | Pages | 5305-22 |
| PubMed ID | 3290851 | Mgi Jnum | J:9239 |
| Mgi Id | MGI:57702 | Doi | 10.1093/nar/16.12.5305 |
| Citation | van Duin M, et al. (1988) Evolution and mutagenesis of the mammalian excision repair gene ERCC-1. Nucleic Acids Res 16(12):5305-22 |
| abstractText | The human DNA excision repair protein ERCC-1 exhibits homology to the yeast RAD10 repair protein and its longer C-terminus displays similarity to parts of the E. coli repair proteins uvrA and uvrC. To study the evolution of this 'mosaic' ERCC-1 gene we have isolated the mouse homologue. Mouse ERCC-1 harbors the same pattern of homology with RAD10 and has a comparable C-terminal extension as its human equivalent. Mutation studies show that the strongly conserved C-terminus is essential in contrast to the less conserved N-terminus which is even dispensible. The mouse ERCC-1 amino acid sequence is compatible with a previously postulated nuclear location signal and DNA-binding domain. The ERCC-1 promoter harbors a region which is highly conserved in mouse and man. Since the ERCC-1 promoter is devoid of all classical promoter elements this region may be responsible for the low constitutive level of expression in all mouse tissues and stages of embryogenesis examined. |
