| Primary Identifier | MGI:3522707 | Allele Type | Transgenic |
| Attribute String | Reporter | Gene | Tg(HK2-luc)253Uku |
| Strain of Origin | (BALB/c x DBA/2)F1 | Is Recombinase | false |
| Is Wild Type | false |
| description | No distinction was made among the 5 transgenic lines lines carrying from 1-2 to 230-240 copies of the same construct; the authors state there were no significant differences among them, whether or not results were normalized to copy number. It was noted that baseline luciferase expression varied inversely with transgene copy number. Mean basal luciferase activities in heart, skeletal muscle and adipose tissue of 4 to 5-week old transgenic mice were 4- to 38-fold higher than in tissues of mice bearing transgenes containing 254 base pairs of the HK2 promoter. Insulin induction in 5 to 6-week old transgenic mice by 4 consecutive glucose injections did not significantly increase luciferase activity in heart or skeletal muscle. |
| molecularNote | This transgenic line carries 15-20 copies of a construct comprising 505 base pairs of 5' flanking DNA and 106 base pairs of untranslated sequence from exon I of the human hexokinase II gene joined to the firefly luciferase coding sequence, which is followed by the SV40 small intron and polyadenylation signal. |
