| First Author | Wacker SA | Year | 2011 |
| Journal | EMBO J | Volume | 30 |
| Issue | 1 | Pages | 43-56 |
| PubMed ID | 21102556 | Mgi Jnum | J:200507 |
| Mgi Id | MGI:5508736 | Doi | 10.1038/emboj.2010.289 |
| Citation | Wacker SA, et al. (2011) RITA, a novel modulator of Notch signalling, acts via nuclear export of RBP-J. EMBO J 30(1):43-56 |
| abstractText | The evolutionarily conserved Notch signal transduction pathway regulates fundamental cellular processes during embryonic development and in the adult. Ligand binding induces presenilin-dependent cleavage of the receptor and a subsequent nuclear translocation of the Notch intracellular domain (NICD). In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. Here, we report the identification and functional characterization of RBP-J interacting and tubulin associated (RITA) (C12ORF52) as a novel RBP-J/CBF-1-interacting protein. RITA is a highly conserved 36 kDa protein that, most interestingly, binds to tubulin in the cytoplasm and shuttles rapidly between cytoplasm and nucleus. This shuttling RITA exports RBP-J/CBF-1 from the nucleus. Functionally, we show that RITA can reverse a Notch-induced loss of primary neurogenesis in Xenopus laevis. Furthermore, RITA is able to downregulate Notch-mediated transcription. Thus, we propose that RITA acts as a negative modulator of the Notch signalling pathway, controlling the level of nuclear RBP-J/CBF-1, where its amounts are limiting. |
