| First Author | Imanishi Y | Year | 2008 |
| Journal | J Biol Chem | Volume | 283 |
| Issue | 36 | Pages | 25091-102 |
| PubMed ID | 18606814 | Mgi Jnum | J:142021 |
| Mgi Id | MGI:3820192 | Doi | 10.1074/jbc.M802981200 |
| Citation | Imanishi Y, et al. (2008) Retinyl ester homeostasis in the adipose differentiation-related protein-deficient retina. J Biol Chem 283(36):25091-102 |
| abstractText | The retinal pigmented epithelium (RPE) plays an essential role in vision, including storing and converting retinyl esters of the visual chromophore, 11-cis-retinal. Retinyl ester storage structures (RESTs), specialized lipid droplets within the RPE, take up retinyl esters synthesized in the endoplasmic reticulum. Here we report studies of mice lacking exons 2 and 3 of the gene encoding adipose differentiation-related protein (Adfp), a structural component of RESTs. We found that dark adaptation was slower in Adfp(Delta2-3/Delta2-3) than in Adfp+/+ mice and that Adfp(Delta2-3/Delta2-3) mice had consistently delayed clearances of all-trans-retinal and all-trans-retinol from rod photoreceptor cells. Two-photon microscopy revealed aberrant trafficking of all-trans-retinyl esters in the RPE of Adfp(Delta2-3/Delta2-3) mice, a problem caused by abnormal maintenance of RESTs in the dark-adapted state. Retinyl ester accumulation was also reduced in Adfp(Delta2-3/Delta2-3) as compared with Adfp+/+ mice. These observations suggest that Adfp plays a unique role in vision by maintaining proper storage and trafficking of retinoids within the eye. |
