| First Author | Fehling HJ | Year | 1997 |
| Journal | Immunity | Volume | 6 |
| Issue | 6 | Pages | 703-14 |
| PubMed ID | 9208843 | Mgi Jnum | J:111526 |
| Mgi Id | MGI:3654369 | Doi | 10.1016/s1074-7613(00)80446-x |
| Citation | Fehling HJ, et al. (1997) Restoration of thymopoiesis in pT alpha-/- mice by anti-CD3epsilon antibody treatment or with transgenes encoding activated Lck or tailless pT alpha. Immunity 6(6):703-14 |
| abstractText | Mice deficient for the pre-TCR alpha (pT alpha) chain cannot form a pre-T cell receptor (TCR) and exhibit a severe defect in early T cell development, characterized by lack of 'beta selection' and impaired generation of double-positive (DP) thymocytes. Here, we demonstrate that intraperitoneal injection of CD3epsilon-specific antibodies into pT alpha-/- x RAG-/- mice or introduction of an activated p56(lck) transgene in pT alpha-/- mice fully restores the number of DP thymocytes, and that expression of a transgenic pT alpha chain lacking its cytoplasmic portion can overcome all developmental defects associated with pT alpha deficiency. These results allow a better definition of the role of pT alpha in pre-TCR signal transduction and provide conclusive evidence that the cytoplasmic tail of pT alpha is not essential for pre-TCR signaling. |
