| First Author | Kataoka K | Year | 1998 |
| Journal | Somat Cell Mol Genet | Volume | 24 |
| Issue | 4 | Pages | 235-43 |
| PubMed ID | 10410677 | Mgi Jnum | J:56483 |
| Mgi Id | MGI:1341004 | Doi | 10.1023/b:scam.0000007125.41715.8d |
| Citation | Kataoka K, et al. (1998) Characterization of a human genomic DNA fragment which rescues defective lipid-linked oligosaccharide synthesis in a mutant G258 cell line isolated from the FM3A mouse mammary carcinoma cell line. Somat Cell Mol Genet 24(4):235-43 |
| abstractText | The G258 mutant cell line, isolated from the FM3A mouse mammary carcinoma cell line, is temperature-sensitive for both cell growth and asparagine-linked glycosylation due to mutation at a single location. The biochemical defect in the G258 mutant resides in the formation of lipid- linked oligosaccharide, presumably in one of the steps of GDP-mannose-dependent mannosylation (Y; Nishikawa, J, Cell. Physiol. 119, 260-266 1984; Y. Nishikawa Biochim. Biophys. Acta 1091, 135-140 1991). In the present study, we transfected human genomic DNA fragments into the G258 mutant by the radiation hybrid method and isolated transformants (KK-I, -3 and -4) which showed recovery from both temperature-sensitive cell growth and asparagine- linked glycosylation. These transformants contained a common Alu-containing human DNA fragment (1.3 kb) which will be used as a marker for isolating the gene that complements the defect of lipid-linked oligosaccharide synthesis in the G258 mutant. |
