| First Author | Li H | Year | 2013 |
| Journal | Transgenic Res | Volume | 22 |
| Issue | 1 | Pages | 169-78 |
| PubMed ID | 22661167 | Mgi Jnum | J:192684 |
| Mgi Id | MGI:5466208 | Doi | 10.1007/s11248-012-9623-1 |
| Citation | Li H, et al. (2013) Expression of biologically active human interferon alpha 2b in the milk of transgenic mice. Transgenic Res 22(1):169-78 |
| abstractText | Interferon alpha 2b (IFNalpha-2b) is an important immune regulator widely used in clinic, for the treatment of chronic hepatitis, hairy cell leukemia, chronic myelogenous leukemia and multiple myeloma, etc. The clinically used IFNalpha-2b is generally produced by E.Coli, which lacks the post-translational O-glycosylation presents on naturally synthesized protein, and has a short serum half-life. In this study, a transgenic cassette pBCN-IFN-pA-CMV-EGFP was constructed, with a 5.2 kb beta-casein regulation fragment from Jersey cow and a 6xHis tagged human Interferon alpha 2b (hIFNalpha-2b) gene fragment. By using pronuclear microinjection technique, transgenic mice were generated and the expression of IFNalpha-2b in the milk was assayed. The hIFNalpha-2b was correctly translated in milk of transgenic mice according to Western blot analysis. The expression level of hIFNalpha-2b was varied among the transgenic mice, and the highest one was about 29.71 mug/L. The recombinant protein exhibited biological activity in vitro by increasing the luminescence value and the MxA gene expression in established WISH cells, and the specific activity is approximately 2.8 x 10(7 )IU/mg. The expression of recombinant hIFNalpha-2b in mammary glands of transgenic mice constitutes an important step towards low-cost and full biological activity production of this protein drug in mammary gland bioreactor. |
