| First Author | Hogmalm A | Year | 2012 |
| Journal | Am J Respir Cell Mol Biol | Volume | 47 |
| Issue | 6 | Pages | 746-58 |
| PubMed ID | 22865624 | Mgi Jnum | J:204048 |
| Mgi Id | MGI:5529444 | Doi | 10.1165/rcmb.2011-0394OC |
| Citation | Hogmalm A, et al. (2012) Role of CXC chemokine receptor-2 in a murine model of bronchopulmonary dysplasia. Am J Respir Cell Mol Biol 47(6):746-58 |
| abstractText | The contribution of neutrophils and CXC chemokines to the pathogenesis of bronchopulmonary dysplasia is not well defined. The transgenic expression of IL-1beta in the pulmonary epithelium causes lung inflammation and disrupts alveolar development in infant mice. To study the hypothesis that CXC chemokine receptor-2 (CXCR2) is a mediator of inflammatory lung injury, we compared lung development in IL-1beta-expressing mice with wild-type (IL-1beta/CXCR2(+/+)) or null (IL-1beta/CXCR2(-/-)) CXCR2 loci. CXCR2 deficiency abolished the transmigration of neutrophils into the alveolar lumen in IL-1beta-expressing mice, but did not alter the number of neutrophils in the parenchyma. The deletion of CXCR2 increased the alveolar chord length and reduced the survival of mice when IL-1beta was expressed from the pseudoglandular to the alveolar stages. The capillary configuration was highly abnormal in both IL-1beta/CXCR2(+/+) and IL-1beta/CXCR2(-/-) lungs, but in very different ways. The cellular area of the parenchyma and the total capillary area of IL-1beta/CXCR2(+/+) and IL-1beta/CXCR2(-/-) mice were smaller than those of control/CXCR2(+/+) and control/CXCR2(-/-) mice, but the ratio of capillary area to cellular area was similar in all four genotypes. When IL-1beta was expressed during the saccular stage, IL-1beta/CXCR2(-/-) mice had smaller alveolar chord lengths and better survival than did IL-1beta/CXCR2(+/+) mice. Independent of the timing of IL-1beta expression, IL-1beta increased alveolar septal thickness in mice with wild-type CXCR2 loci, but not in CXCR2 null mice. Depending on the developmental stage at the time of the inflammatory insult, inhibition of the CXCR2 pathway may exert opposite effects on alveolar septation in the neonatal lung. |
