| First Author | Utsunomiya-Tate N | Year | 1997 |
| Journal | FEBS Lett | Volume | 416 |
| Issue | 3 | Pages | 312-6 |
| PubMed ID | 9373176 | Mgi Jnum | J:43801 |
| Mgi Id | MGI:1098956 | Doi | 10.1016/s0014-5793(97)01232-5 |
| Citation | Utsunomiya-Tate N, et al. (1997) Tissue specific variants of glutamate transporter GLT-1. FEBS Lett 416(3):312-6 |
| abstractText | cDNA cloning of glutamate transporter GLT-1 from mouse brain and liver has revealed that 5'-ends of the messages are different between brain and liver, In addition, one of the GLT-1 cDNAs isolated from liver has been found to possess a 3'-end different from those of the others, Reverse transcription polymerase chain reaction (RT-PCR) amplification using primers specific for altered 5'-ends has confirmed that brain and liver messages possess their own. Specific 5'-ends, Both of the two 3'-ends have been demonstrated by RT-PCR to be present not only in liver but also in brain, indicating both brain and Liver GLT-1 possess two types of 3'-ends, Although functional properties are not changed by the alteration of N-termini and C-termini when expressed in Xenopus laevis oocytes, co- expression of two liver type GLT-1 with different C- termini (mGLT-1A and mGLT-1B) has been found to result in the increase in V-max of transport without changing K-m, These results suggest the tissue specific alternative splicing at 5'-ends of GLT-1 messages and the interesting association of spliced variants with different C-termini. (C) 1997 Federation of European Biochemical Societies. |
