| First Author | Nonnenmacher H | Year | 1994 |
| Journal | Biochem Biophys Res Commun | Volume | 204 |
| Issue | 3 | Pages | 1299-304 |
| PubMed ID | 7980608 | Mgi Jnum | J:21492 |
| Mgi Id | MGI:69454 | Doi | 10.1006/bbrc.1994.2604 |
| Citation | Nonnenmacher H, et al. (1994) Contrary results on mouse hepatitis virus type 3 susceptibility in A/J mouse hepatocytes of phosphatidylserine treatment and of a hypercholesterolaemic diet: no correlation with membrane fluidity levels. Biochem Biophys Res Commun 204(3):1299-304 |
| abstractText | The aim of this study was to examine whether or not membrane fluidity directly influences infection by enveloped viruses, and, more precisely here, the susceptibility of A/J mouse hepatocytes to Mouse Hepatitis Virus type 3 (MHV3). We therefore studied, in parallel, the effects on hepatocyte membrane fluidity and on intracellular viral titre of two treatments, i) a hypercholesterolaemic diet to increase the hepatocyte membrane cholesterol content, ii) direct phosphatidylserine incorporation into hepatocyte membrane. Membrane fluidity was monitored on isolated hepatocytes by fluorescence anisotropy with TMA-DPH, and the viral titre was determined by plaque assay. The results clearly demonstrate that membrane fluidity is not directly involved in viral infection mechanisms. |
