Primary Identifier | MGI:4357768 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Asz1 |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exons 1 and 2, which encode the transcriptional start site and the initiation ATG codon, were replaced by a PgkHPRT expression cassette. Correct targeting in HPRT-null ES cells was confirmed by Southern blotting. Gene inactivation was confirmed by immunoblot analysis on homozygous testes extracts. |