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Publication : Nucleotide sequence and tissue distribution of mouse transforming growth factor-alpha.

First Author  Vaughan TJ Year  1992
Journal  Biochim Biophys Acta Volume  1132
Issue  3 Pages  322-4
PubMed ID  1420315 Mgi Jnum  J:41341
Mgi Id  MGI:893773 Doi  10.1016/0167-4781(92)90170-5
Citation  Vaughan TJ, et al. (1992) Nucleotide sequence and tissue distribution of mouse transforming growth factor-alpha. Biochim Biophys Acta 1132(3):322-4
abstractText  Transforming growth factor-alpha (TGF alpha) is secreted into the medium of the Moloney murine sarcoma virus-transformed 3T3 cell line, 3B11-1C. Using reverse transcription-polymerase chain reaction (RT-PCR), we have amplified, cloned and sequenced a cDNA fragment from this cell line which encodes the full protein-coding region of the mouse TGF alpha precursor. The deduced amino acid sequence (159 residues) differs from that of human TGF alpha at 13 sites and from that of rat at 3 sites. In the mouse, TGF alpha transcripts were detected in a variety of normal adult tissues including two previously unreported sites: the preputial glands and the bladder.
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