First Author | Mori Y | Year | 2013 |
Journal | Biochem Biophys Res Commun | Volume | 435 |
Issue | 1 | Pages | 113-9 |
PubMed ID | 23624502 | Mgi Jnum | J:222141 |
Mgi Id | MGI:5644013 | Doi | 10.1016/j.bbrc.2013.04.051 |
Citation | Mori Y, et al. (2013) Small GTPase Rab39A interacts with UACA and regulates the retinoic acid-induced neurite morphology of Neuro2A cells. Biochem Biophys Res Commun 435(1):113-9 |
abstractText | We screened for a Rab39-specific effector by performing a yeast two-hybrid assay with GTP-locked Rab39A/B as the bait and identified UACA (uveal autoantigen with coiled-coil domains and ankyrin repeats) as a specific Rab39A/B-binding protein. Deletion analysis revealed that a C-terminal coiled-coil domain of UACA functions as a GTP-dependent Rab39-binding domain. shRNA-mediated knockdown of endogenous Rab39A or UACA in mouse neuroblastoma Neuro2A cells resulted in a change in retinoic acid-induced neurite morphology from a multipolar morphology to a bipolar morphology. Taken together, these findings indicate that UACA functions as a Rab39A effector in the retinoic acid-induced differentiation of Neuro2A cells. |