| Primary Identifier | MGI:7571537 | Allele Type | Targeted |
| Gene | Prkab1 | Transmission | Germline |
| Strain of Origin | C57BL/6NTac | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | An FRT site and a lox5171 site flanked neomycin resistance gene cassette were inserted into intron 1 and serine codon 108 (AGC) in exon 2 and 3 was changed to alanine (GCC) (p.S108A). A loxP site, a second FRT site, a copy of the gene from exon 2 onward with intron 2 removed (and containing the p.S108A mutation in the fused exon 2-3), and an F3 site flanked puromycin resistance gene cassette and a second loxP site were inserted downstream of the gene. Subsequent Cre-mediated recombination removed the neo cassette, the duplicated sequence and puro cassette. This allele express transcripts containing the mutation on the endogenous exon structure. The mutation prevents phosphorylation of the residue in the encoded peptide. |
