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Allele : Col1a1<tm6(tetO-Nanog)Hoch> collagen, type I, alpha 1; targeted mutation 6, Konrad Hochedlinger
Primary Identifier  MGI:6368532 Allele Type  Targeted
Attribute String  Inducible, Inserted expressed sequence Gene  Col1a1
Transmission  Germline Strain of Origin  (C57BL/6 x 129S4/SvJae)F1
Induced With  doxycycline/tetracycline Is Recombinase  false
Is Wild Type  false
description  The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae.
molecularNote  A targeting vector was designed to insert an FRT-flanked PGK-Neo-pA 500 bp downstream of the 3' UTR of the Col1a1 locus. The FRT-flanked PGK-Neo-pA in the 3' UTR of the Col1a1 locus was replaced by co-injection of a tetO-Nanog (Nanog homeobox) plasmid and flpe recombinase. The tetO-Nanog plasmid contained a splice acceptor-double polyA sequence and the tetracycline responsive element (tetO) upstream of the Nanog gene.
  • mutations:
  • Insertion
  • synonyms:
  • Col1a1<tetO-Nanog>,
  • Col1a1<tetO-Nanog>
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1 Feature

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Genome

1 Expresses

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0 Mutation Involves

Phenotype

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