| First Author | Kim JY | Year | 2005 |
| Journal | Biochem Biophys Res Commun | Volume | 326 |
| Issue | 1 | Pages | 36-44 |
| PubMed ID | 15567149 | Mgi Jnum | J:83859 |
| Mgi Id | MGI:2663940 | Doi | 10.1016/j.bbrc.2004.10.200 |
| Citation | Kim JY, et al. (2004) Cloning, expression, and differentiation-dependent regulation of SMAF1 in adipogenesis. Biochem Biophys Res Commun 326(1):36-44 |
| abstractText | With the aim of identifying novel molecular pathways in the adipocyte, we conducted differential screening of DNA filter arrays with probes from 3T3-L1 preadipocytes and adipocytes, and discovered a novel 0.7kb transcript we term small adipocyte factor 1 (SMAF1). SMAF1 encodes a wholly novel 10kDa protein. Transfection and localization studies of a SMAF1-EGFP fusion construct indicate nuclear localization, suggestive of a possible regulatory role. Northern blot analysis of various murine tissues indicates adipose tissue-restricted expression, and fractionation of adipose tissue reveals that SMAF1 is expressed soley in adipocytes and not in the stromal-vascular cell population. Northern blot analysis of brown and white adipogenic conversion reveals that expression of SMAF1 closely parallels emergence of an adipocyte phenotype and that TNFalpha-mediated dedifferentiation of 3T3-L1 adipocytes results in a rapid decline of SMAF1 transcript. These data indicate that SMAF1 is closely tied to the adipocyte phenotype and predict a novel and possibly regulatory role for this gene in adipocyte function. |
