| First Author | Ren X | Year | 2009 |
| Journal | Structure | Volume | 17 |
| Issue | 3 | Pages | 406-16 |
| PubMed ID | 19278655 | Mgi Jnum | J:245341 |
| Mgi Id | MGI:5918198 | Doi | 10.1016/j.str.2009.01.012 |
| Citation | Ren X, et al. (2009) Hybrid structural model of the complete human ESCRT-0 complex. Structure 17(3):406-16 |
| abstractText | The human Hrs and STAM proteins comprise the ESCRT-0 complex, which sorts ubiquitinated cell surface receptors to lysosomes for degradation. Here we report a model for the complete ESCRT-0 complex based on the crystal structure of the Hrs-STAM core complex, previously solved domain structures, hydrodynamic measurements, and Monte Carlo simulations. ESCRT-0 expressed in insect cells has a hydrodynamic radius of RH = 7.9 nm and is a 1:1 heterodimer. The 2.3 Angstroms crystal structure of the ESCRT-0 core complex reveals two domain-swapped GAT domains and an antiparallel two-stranded coiled-coil, similar to yeast ESCRT-0. ESCRT-0 typifies a class of biomolecular assemblies that combine structured and unstructured elements, and have dynamic and open conformations to ensure versatility in target recognition. Coarse-grained Monte Carlo simulations constrained by experimental RH values for ESCRT-0 reveal a dynamic ensemble of conformations well suited for diverse functions. |
