| First Author | Lee J | Year | 2011 |
| Journal | BMC Neurosci | Volume | 12 |
| Pages | 9 | PubMed ID | 21247490 |
| Mgi Jnum | J:288544 | Mgi Id | MGI:6433157 |
| Doi | 10.1186/1471-2202-12-9 | Citation | Lee J, et al. (2011) Non-invasive quantification of brain tumor-induced astrogliosis. BMC Neurosci 12:9 |
| abstractText | BACKGROUND: CNS injury including stroke, infection, and tumor growth lead to astrogliosis, a process that involves upregulation of glial fibrillary acidic protein (GFAP) in astrocytes. However, the kinetics of astrogliosis that is related to these insults (i.e. tumor) is largely unknown. RESULTS: Using transgenic mice expressing firefly luciferase under the regulation of the GFAP promoter (GFAP-luc), we developed a model system to monitor astrogliosis upon tumor growth in a rapid, non-invasive manner. A biphasic induction of astrogliosis was observed in our xenograft model in which an early phase of activation of GFAP was associated with inflammatory response followed by a secondary, long-term upregulation of GFAP. These animals reveal GFAP activation with kinetics that is in parallel with tumor growth. Furthermore, a strong correlation between astrogliosis and tumor size was observed. CONCLUSIONS: Our results suggest that non-invasive, quantitative bioluminescent imaging using GFAP-luc reporter animal is a useful tool to monitor temporal-spatial kinetics of host-mediated astrogliosis that is associated with glioma and metastatic brain tumor growth. |
