| First Author | Jaramillo M | Year | 1991 |
| Journal | J Biol Chem | Volume | 266 |
| Issue | 16 | Pages | 10446-51 |
| PubMed ID | 2037592 | Mgi Jnum | J:11203 |
| Mgi Id | MGI:59644 | Doi | 10.1016/s0021-9258(18)99245-8 |
| Citation | Jaramillo M, et al. (1991) Multiple mRNAs encode the murine translation initiation factor eIF-4E. J Biol Chem 266(16):10446-51 |
| abstractText | All eukaryotic cellular mRNAs (except organellar) possess at their 5' end the structure m7GpppX (where X is any nucleotide) termed the cap. The cap structure facilitates the melting of mRNA 5' secondary structure through the action of initiation factor-4F (eIF-4F) in conjunction with eIF-4B. eIF-4F consists of three subunits of which one, eIF-4E (eIF-4E has recently been designated eIF-4 alpha according to the Nomenclature Committee of the International Union of Biochemistry (NC-IUB) (Safer, B. (1989) Eur. J. Biochem. 186, 1-3)), contains the cap binding site. Several lines of evidence suggest that eIF-4E regulates the rate of translation initiation. Consequently, changes in cellular eIF-4E levels could control growth and differentiation. To investigate the possibility that eIF-4E expression is regulated, we studied the pattern of eIF-4E expression in several cell lines. Here, we show the existence of multiple mRNAs for eIF-4E that are generated by differential polyadenylation. In addition, we show tissue-specific differences in eIF-4E mRNA expression and utilization of polyadenylation sites. |
