| Primary Identifier | MGI:6158670 | Allele Type | Targeted |
| Attribute String | Conditional ready, Null/knockout, Reporter | Gene | Bsph1 |
| Transmission | Germline | Strain of Origin | C57BL/6NSlc-Tg(CAG-EGFP,Acr-EGFP)2Osb |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | This knockout-first reporter allele with conditional potential was generated by electroporation of embryonic stem cells with the AsiSI-linearized targeting vector HTGRS6004_A_H09, obtained from KOMP. The vector introduced a FRT site; the mouse engrailed 2 (En2) intron, splice acceptor and exon; an internal ribosomal entry site (IRES); the lacZ coding sequence with a polyadenylation signal (p[A]); a loxP site; a neomycin resistance cassette with the human beta-actin promoter and a p[a]; a second FRT site; and a second loxP site into intron 3 and a third loxP site into exon 5. |
