| First Author | Kenswil KJG | Year | 2021 |
| Journal | Cell Stem Cell | Volume | 28 |
| Issue | 4 | Pages | 653-670.e11 |
| PubMed ID | 33561425 | Mgi Jnum | J:313383 |
| Mgi Id | MGI:6710421 | Doi | 10.1016/j.stem.2021.01.006 |
| Citation | Kenswil KJG, et al. (2021) Endothelium-derived stromal cells contribute to hematopoietic bone marrow niche formation. Cell Stem Cell 28(4):653-670.e11 |
| abstractText | Bone marrow stromal cells (BMSCs) play pivotal roles in tissue maintenance and regeneration. Their origins, however, remain incompletely understood. Here we identify rare LNGFR(+) cells in human fetal and regenerative bone marrow that co-express endothelial and stromal markers. This endothelial subpopulation displays transcriptional reprogramming consistent with endothelial-to-mesenchymal transition (EndoMT) and can generate multipotent stromal cells that reconstitute the bone marrow (BM) niche upon transplantation. Single-cell transcriptomics and lineage tracing in mice confirm robust and sustained contributions of EndoMT to bone precursor and hematopoietic niche pools. Interleukin-33 (IL-33) is overexpressed in subsets of EndoMT cells and drives this conversion process through ST2 receptor signaling. These data reveal generation of tissue-forming BMSCs from mouse and human endothelial cells and may be instructive for approaches to human tissue regeneration. |
