| First Author | Yu H | Year | 2012 |
| Journal | FEBS Lett | Volume | 586 |
| Issue | 10 | Pages | 1466-71 |
| PubMed ID | 22673512 | Mgi Jnum | J:185498 |
| Mgi Id | MGI:5429084 | Doi | 10.1016/j.febslet.2012.03.067 |
| Citation | Yu H, et al. (2012) alpha-Actinin4 nuclear translocation mediates gonadotropin-releasing hormone stimulation of follicle-stimulating hormone beta-subunit gene transcription in LbetaT2 cells. FEBS Lett 586(10):1466-71 |
| abstractText | Gonadotropin-releasing hormone (GnRH) regulates the synthesis and secretion of follicle-stimulating hormone (FSH) by stimulating the transcription of Fshbeta gene. Our iTRAQ quantitative proteomics result showed that the abundance of alpha-actinin4 (ACTN4) increased in the nuclei of LbetaT2 cells upon GnRH induction. Using RNA interference, reverse transcription and real-time PCR, luciferase and transient transfection assays, we proved that ACTN4 is involved in the regulation of mouse Fshbeta gene (mFshbeta) transcription and its C-terminal calmodulin (CaM)-like domain is crucial for this process. Our study suggests that ACTN4 nuclear translocation mediates GnRH stimulation of mFshbeta gene transcription. |
