Primary Identifier | MGI:2386105 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Smarca4 |
Transmission | Germline | Strain of Origin | 129S/Sv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A neomycin selection cassette replaced sequence encoding part of the DNA-dependent ATPase domain. The deleted region encoded helicase motif 1, which contains the ATP binding site, and helicase motif 1a. The allele was expected to encode a chimeric protein, though while Northern blot analysis identified transcripts containing both endogenous and neo transgene elements, protein was undetected by Western blot analysis. |