| First Author | Horton MA | Year | 1996 |
| Journal | J Leukoc Biol | Volume | 60 |
| Issue | 2 | Pages | 285-94 |
| PubMed ID | 8773591 | Mgi Jnum | J:34899 |
| Mgi Id | MGI:82354 | Doi | 10.1002/jlb.60.2.285 |
| Citation | Horton MA, et al. (1996) Cloning of the murine eosinophil peroxidase gene (mEPO): characterization of a conserved subgroup of mammalian hematopoietic peroxidases. J Leukoc Biol 60(2):285-94 |
| abstractText | The mouse eosinophil peroxidase (mEPO) gene was cloned by screening a random-primed bone marrow cDNA library at reduced criteria using a hEPO cDNA. An mEPO cDNA was subsequently used to isolate the mEPO gene from a lambda-genomic library. The mEPO gene displays a high degree of conservation with its human homologue: the transcription units are approximately the same size, conserve the relative size and position of the 12 exons associated with each gene, and at a nucleotide level the mouse and human EPO genes are 86% identical in the protein coding regions and 66% identical in the 3'-untranslated trailer regions. This strong conservation extends to the encoded proteins which show approximately 90% amino acid identity. Expression of the mEPO gene is restricted to tissues containing eosinophil progenitor cells (e.g., bone marrow and spleen), a pattern similar to the expression of another murine eosinophil granule protein, major basic protein. |
