| Primary Identifier | MGI:3624796 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | Rb1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was used to insert I740A, N744A, M748A point mutations in the LXCXE binding site. Sequencing of exon 22 confirmed successful recombination. Analysis of mutant fibroblasts confirmed that protein levels were equivalent to wild-type cells. Crossing with mice expressing cre recombinase in the germline removed a floxed neo included in the vector. These substitutions are predicted to make the leucine and cysteine residues of the LXCXE motif a looser fit than the endogenous cleft. |
