| First Author | Miyake K | Year | 1998 |
| Journal | J Immunol | Volume | 161 |
| Issue | 3 | Pages | 1348-53 |
| PubMed ID | 9686597 | Mgi Jnum | J:48825 |
| Mgi Id | MGI:1275858 | Doi | 10.4049/jimmunol.161.3.1348 |
| Citation | Miyake K, et al. (1998) Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression. J Immunol 161(3):1348-53 |
| abstractText | RP105 is a leucine-rich repeat molecule that is expressed on mouse B cells and transmits a growth-promoting signal. An anti-RP105 Ab precipitated additional molecules as well as RP105. These molecules were found to be a mouse homologue of chicken MD-1. Chicken MD-1 was previously isolated as a v-myb-regulated gene, since its transcription increases rapidly after v-myb induction. Mouse MD-1, when transiently expressed as an epitope-tagged protein, is secreted in culture fluid but tethered to the cell surface by coexpressed RP105. An association of these molecules was confirmed by immunoprecipitation with the anti-RP105 Ab and subsequent probing of the epitope tag on MD-1. Moreover, MD-1 has an effect on the expression of RP105. In transient transfection of RP105, the percentage of RP105-positive cells increased more than twice with the coexpression of MD-1. The stable expression of MD-1 conferred approximately a sevenfold increase in cell surface RP105 on a cell line that expresses RP105 alone. Thus, MD-1 is physically associated with RP105 and is important for efficient cell surface expression. |
