| Primary Identifier | MGI:3033425 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Dph1 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae-Tg(Prm-cre)70Og |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 7 to 12 as well as part of exon 13 were replaced with a floxed neomycin resistance gene. In the process, exon 1 of Ovca2 which is located between exons 12 and 13 was also deleted. Targeting took place in PC3 ES cells homozygous for Tg(Prm-cre)70Og, which expresses Cre recombinase under control of the male-germline specific protamine promoter. Cre mediated excision subsequently removed the neomycin cassette upon transmission from male chimeric mice. RT-PCR indicated that no full length transcripts were produced but transcripts were produced from exons 1-6. |
