| First Author | Sharma HS | Year | 1995 |
| Journal | Biochim Biophys Acta | Volume | 1260 |
| Issue | 2 | Pages | 235-8 |
| PubMed ID | 7841203 | Mgi Jnum | J:22539 |
| Mgi Id | MGI:70402 | Doi | 10.1016/0167-4781(94)00221-n |
| Citation | Sharma HS, et al. (1995) Nucleotide sequence and expression of the porcine vascular endothelial growth factor. Biochim Biophys Acta 1260(2):235-8 |
| abstractText | We cloned and sequenced two cDNAs encoding the angiogenic, vascular endothelial growth factor (VEGF) from the porcine heart. Deduced amino acid sequence of the clone pPVE-18 and pPVE-5 predicted 164 (VEGF164), and 120 (VEGF120) residues of VEGF, respectively, with a putative N-terminal signal sequence of 26 amino acids. The porcine VEGF is shorter by one amino acid as compared to human VEGF, but a potential glycosylation site is present at Asn-74. PCR detection, and verification of the identity of the PCR products by Southern hybridization, confirmed wide expression of VEGF in different porcine tissues. Northern blot analysis with a radiolabeled porcine specific VEGF probe, showed one major (3.9 kb) and one minor (1.7 kb) mRNA species expressed in all four chambers of the heart. |
